Healthier platelets ? Platelets in large numbers?
The solution is the APGFpro® PRP Bag System
What is Platelet-Rich-Plasma (PRP)?
PRP is a platelet concentrate of autologous blood in a sufficient volume of plasma. Unfortunately, even after three decades of using platelet-rich plasma in various medical settings, there is still no universally accepted definition for PRP. Many PRP users think that PRP is given at any concentration compared to baseline as long as an "enrichment" sequence is performed. The following parameters are generally accepted.
What is effective PRP?
Platelet counts in the blood range from 150,000 to 350,000 per microliter of blood (with an average of 200,000/µl). Based on recorded effective bone and soft tissue healing treatments, it is concluded that at least 1,000,000 platelets per microliter must be counted to obtain effective PRP. Whether a concentration below or above this value achieves optimal results is questioned.
Why do you need healthy platelets?
1,000,000 platelets/µl is achieved by concentrating these 4-5 times above baseline. For effective, sustainable PRP treatment, the "health" of these platelets must be as good as possible. Don't forget that platelets contain the growth factors that exert their effectiveness locally in the tissue
Why do we centrifuge twice?
To achieve a platelet count of 1,000,000/µl, we need to gently separate RBCs and WBCs from platelet-containing plasma. This is done gently in the first centrifugation cycle. In a second centrifugation, the platelets are concentrated in the plasma. The platelets accumulate at the bottom of the second bag. Thus, the PRP (rich) and the PPP (poor) are generated with this second spin. They determine the concentration of the PRP during collection.
PRP is a blood product. Therefore, APGFpro® follows the same procedures, production concepts and standards of the blood banks for blood collection and preparation. The practitioner receives healthy and highly concentrated PRP for his customers. The gentle PRP bag technology minimizes damage to platelets during distribution. The growth factors are only activated in the body after 30 days at the earliest.
The biggest challenge for a PRP practitioner is not only to collect enough platelets, but also enough healthy platelets in the applicator. Often the challenges are relatively clear:
Platelets are very fragile cells. They are quickly damaged and growth factors are released too early. This can make PRP treatment more ineffective.
On the one hand, the hard walls of the classic tubes, and on the other hand, the high speeds during centrifugation, are a problem for platelets. Classic PRP tubes are hard tubes made of various polymers or glass. When the blood is centrifuged, the platelets collide against this wall as well as against the leukocytes, which are much larger.
Due to the high rotation speeds, high centrifugal forces also act on the cells and thus lead to large shear forces, which increase the negative effect.